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This was evidenced by RBC Prdx2 in G6PD-deficient infants, with a higher proportion shown to maintain the oxidized state and are poorly reactivated after peroxide challenge (20)

2.5.2 cDNA synthesis and RT-qPCR Reverse transcription was conducted with 40 ng of RNA per sample using the Reverse Transcription Master Mix Kit (Fluidigm Corporation, San Francisco, California, USA), following the manufacturers instructions

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