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7 L of RNA was added to the RT and buffer mixture prepared with random hexamers and oligoDT primers as per kit instructions, and rtPCR carried out with the following programme: 10 min at 25C, 30 min at 55C and 5 min at 85C

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doi: 10.1038/nature25172 23 WenXWangHGZhangMNZhangMHWangHYangXZ

Interestingly, the study did find that sublingual GSH led to slightly greater glutathione levels compared to NAC, but both did increase levels

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