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Mice were first anesthetized with tribromoethanol (TBE) and then perfused transcardially with cold 0.9% saline, followed by cold fixative (4% paraformaldehyde in borate buffer, pH 9.5) for 20 min
The pace of dose increases varies by individual
Para que esto se traduzca en una prdida de grasa corporal, no solo tendremos que poner la grasa a disposicin de la clula para que esta la use como sustrato metablico , sino tambin debemos generar un dficit calrico (para ir perdiendo grasa paulatinamente) a travs de la alimentacin y la actividad fsica
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