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Absorption of the His-tagged protein is performed at neutral to slightly alkaline pH to prevent protonation and loss of binding capacity of the weakly basic histidine imidazole groups

Zinc also modulates key nodes within the UPR: PERK activity depends on zinc-binding domains, zinc influences eIF2 phosphorylation and ER chaperones such as BiP and CHOP are regulated by zinc availability 123

To address these factors, models were run individually, adjusting for (1) age, (2) sex, (3) gray-to-white matter fraction, (4) SNR, (5) antidepressant status and (6) pretreatment order

Pharmacol Biochem Behav 46 : 423426 Moreau JL, Jenck F, Martin JR, Mortas P, Haefely WE

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