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RNA quality was assessed by 1% agarose gel electrophoresis, and concentration was determined using a NanoDrop spectrophotometer

defining the binding of VNAR-D1 and VNAR-C4 on TNF- and assessing the functional implications of those interactions within a defined biological context, we recognize that a deeper mechanistic and functional dissection of TNFR1 and TNFR2 signaling, particularly in response to tmTNF-, will be of great interest

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