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Figure 28: Photograph of the gastrointestinal researchers at Bispebjerg Hospital in 1975, credit: Figure 1.3.3, Holst 2024

Further, choline is a precursor for the neurotransmitter acetylcholine (13)

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J Med Life 12(2):113 Calvez V, Puca P, Di Vincenzo F, Del Gaudio A, Bartocci B, Murgiano M, Iaccarino J, Parand E, Napolitano D, Pugliese D, Gasbarrini A (2025) Novel insights into the pathogenesis of inflammatory bowel diseases

Both tissue and cellular RNA were extracted using the TRIzol reagent (15596026CN, Invitrogen) following the manufacturers protocol