Epifluorescent pictures (n = 8 per coverslip) were taken at a 40 magnification (0.085 m/pixel) with the use of a Nikon Eclipse 50i epifluorescence microscope (Nikon, Japan) and NIS-Elements Microscope Imaging Software (Nikon)
Iron dose-dependently downregulates the expression of proteins in the Wnt pathway and suppresses of Wnt reporter gene TopFlash transcription
The lungs were lavaged three times using 0.8 ml Dulbecco's phosphate-buffered saline (DPBS) and ~90% of the lavage volume was collected
Sci Rep 11(1):19096 [DOI] [PMC free article] [PubMed] [Google Scholar] Mazzilli R, Medenica S, Di Tommaso A, Fabozzi G, Zamponi V, Cimadomo D, Rienzi L, Ubaldi F, Watanabe M, Faggiano A (2023) The role of thyroid function in female and male infertility: a narrative review
When combined with correct pen use and safe storage, these practices create a smoother treatment experience and more reliable results