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Cells were incubated with 1:100 anti-rabbit GLUT1 (Abcam, ab115730) antibody in BD Stain Buffer (BSA) (BD Biosciences) 30 min at room temperature after 20 min on ice to block nonspecific binding, followed by incubation for 30 min at room temperature with 1:200 Alexa Fluor 647 (Abcam, ab150075)

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