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For the aortic samples from aged mice, first, the aortas from different treatment groups were excised and other tissues were removed, leaving only the aortic endothelium

We found that BSO reduced NPT content under CHT stress, and improved CHT accumulation in tomatoes, suggesting that glutathione promoted CHT metabolism in tomato plants

The samples were centrifuged (16,000 g, 5 min, 25 C), and the absorbance was measured at 405 nm

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