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Materials and methods Drosophila stocks The following transgenes were used: dpp-Gal4 77 , ms1096-Gal4 78 , nub-Gal4 79 , hh-Gal4 (BL#45169), c765-Gal4 80 , UAS-ttvRNAi (VDRC#4871), UAS-smoRNAi (VDRC#9542), UAS-smo PKA 35, UAS-ptc (BL#5817), UAS-dpp (BL#1486), UAS-diap1 81 , UAS-droncRNAi 82 , UAS-driceRNAi (VDRC#28065), UAS-P35 (BL#5073), UAS-dad 28 , UAS-bsk DN (BL#6409), UAS-hepRNAi (VDRC#47507), UAS-hidRNAi (a gift from Lei Xue)

J Transl Med (2020) 18:387

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In vitro research refers to experimental procedures conducted outside of living organisms within controlled laboratory environments