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These sequences were then run through an R script (Supplementary Materials, bash_DADA2 + IDTAXA_workflow_Illumina-PairedEnd_V1V3.R) which trimmed reverse reads to 260 bp, removed forward reads exceeding a maximum expected error of three, and truncated reverse reads exceeding a maximum expected error of six
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In states of obesity and metabolic dysfunction, NNMT is often overexpressed in adipose tissue, depleting nicotinamide and reducing NAD+ availability [2]
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