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Illustrating the complexity of the events that should be involved, for the counteracted effects of insulin-over dose [62], this can be the recovery of both insulin pathways (i.e., prominent counteraction of calcification of liver blood vessels [62] requires both insulin pathways inhibited [270,271])

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Blood samples were collected from the tail tip every 1020 minutes for the immediate measurement of blood glucose, and a 20% glucose solution was infused as needed to maintain blood glucose levels within the euglycemic range

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