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Always handle research compounds in accordance with applicable laws, institutional guidelines, and good laboratory practice

Antibodies The following antibodies were used: antiroGFP2 (this study), antiPgk1 (Invitrogen), antiTom70 (Bien et al , 2010), antiErv1 and antiMia40 (Bien et al , 2010), antiMrpl36 (Gruschke et al , 2010), antiMrpl40 (Gruschke et al , 2010), antiAct1 (Novus Biologicals) and antiGlr1 (antibodies online)

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Key Considerations Dosage calculation : Always use GSH equivalents to avoid double-counting

The GSH/GSSG ratio can be measured in specific organelles using redox-sensitive green fluorescent proteins (roGFPs), particularly those fused to the GSH-specific Grx1 protein 7