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The sections were blocked with 5% goat serum (Jackson ImmunoResearch, 005-000-121) in antibody diluent (Dako) for 30 min and incubated overnight at 4 C with following antibodies: insulin, glucagon, -H2AX, MOTS-c or IL-1 (1:100 of antibody concentration)

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Most clients at Oliva start to notice visible improvement in skin tone after 2-3 sessions