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Collectively, our findings demonstrate that the GPX4 inhibition by RSL3 in PTC cells induces cell death, ferroptosis, lipid peroxidation, increased intracellular ROS burden, and significantly impairs thyroid tumor cell migration, 3D PTC spheroid fotmation and results in reduced spheroid viability in vitro

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NRF2 expression also induces the expression of NQO1, heme-oxygenase 1 (HO1), and FTH1, affecting intracellular iron metabolism, and these processes can be inhibited by p62 activation (57)