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Characterization of antigen A and antigen B antigens The identity and purity of all proteins were confirmed using LC/MS, antibody binding by biolayer interferometry (Octet Red96) or Western blotting, SDS-PAGE under reducing and non-reducing conditions, analytical size exclusion chromatography, and LAL endotoxin measurements following previously described methodology 52

doi: 10.1016/j.jand.2014.01.009 43

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