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MSfragger 4.3 and IonQuant 1.11.11, with carbamidomethyl as a variable modification on cysteine (up to 3 instances), and both the Oxo and the Thio adducts as variable modifications on cysteine, lysine, histidine and tyrosine (up to one instance)

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Its sequence (HXQGTFTSDYSIXLDEEAQAAFIEYLIAGGPSSGAPPPS-NH) incorporates key structural modificationsincluding a C18 fatty diacid chain for extended half-lifethat distinguish it mechanistically from first-generation incretin-based research peptides

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