doi: 10.1074/jbc.M802331200
The procedure is straightforward, safe and usually takes just a few minutes
How AOD-9604 Fits Into Comprehensive Weight Loss Programs Peptide therapy is most effective when viewed as one piece of a larger clinical puzzle
Briefly, cells plated at a density of 2 10 5 cells/cm 2 in 12-well tissue culture-treated polystyrene plates, were first grown for 2 weeks in Williams E medium (Invitrogen, Cergy-Pontoise, France) supplemented with 10% (vol/vol) fetal calf serum (FCS) (Cytiva, Velizy-Villacoublay, France), 100 IU/ml penicillin, 100 g/ml streptomycin, 5 g/ml bovine insulin (SigmaAldrich, Saint-Quentin-Fallavier, France), 2 mM glutamine (Invitrogen) and 50 M hydrocortisone hemisuccinate (Upjohn, Paris La Dfense, France)
FtMt deficiency has been shown to cause mitochondrial iron overload and oxidative stress, triggering ferroptosis through enhanced mitophagy (Wang et al., 2022b)