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The lysis of bacterial cells and denaturation of double-stranded DNA within the AGMs were carried out at room temperature for 30 min with 50 L of an alkaline solution, which was Buffer D2 of the REPLI-g UltraFast Mini Kit (Qiagen)

Pentosidine, a specific AGE, is closely associated with oxidative stress, with accelerated production observed in oxidative stress-related diseases (85)

e forced swim test was done as previously described61

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