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The key steps involve using proper supplies, following sterile technique, mixing gently, and storing correctly

Part of the spinal cord gray and white matter was used for analysis under light microscopy (magnification 300)

Recordings of brain swelling were performed in rats before sacrifice after complete calvariectomy was performed ( Rats were laparatomized before sacrifice for the corresponding presentation of the peripheral vessels (azygos vein, superior mesenteric vein, portal vein, inferior caval vein, and abdominal aorta)

The grinding sensation during certain movements had decreased significantly

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