The enzyme concentration was subsequently determined by UVVis spectroscopy using an extinction coefficient of 51,340 Mcm, which was calculated using Expasy ProtParam ( The oxygen consumption assay was performed using an oxygraph (Hansatech Instruments), which monitors oxygen uptake or evolution in real time using an S1 Clark-type electrode
Stork T, Engelen D, Krudewig A, Silies M, Bainton RJ, Klambt C
Cleared lysates were mixed with protein-loading buffer and boiled at 95 C for 5 min
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There is, however, a synthetic analog of TH4 called TB-500, which has been investigated as a treatment for neurodegenerative conditions like multiple sclerosis, but its use is still largely experimental [4]