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Coupling of the Fmoc-SetCys-OH amino acid : To a solution of Fmoc-SetCys-OH (44.8 mg, 0.100 mmol, 2 equiv) in DMF (2.5 mL) were successively added HOBtH 2 O (15.3 mg, 0.100 mmol, 2 equiv) and DIC (15.6 L, 0.100 mmol, 2 equiv) and the mixture was stirred at rt for 2 min

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It eliminates the need for multiple samples and reduces the chance of errors that can occur with some other tests