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Five microliters of each cDNA sample was added to a 20 l PCR mixture containing 12.5 l of the iQ SYBR Green supermix, 0.5 l of specific primers ACT1, SAP2, HWP1, and EAP1 (Invitrogen Life Technologies Inc., Burlington, ON, Canada), and 7 l of RNase/DNase-free water (MP Biomedicals, Solon, OH, USA)