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Labeling lysosomes with LysoTracker Red and immunostaining In order to visualize acidic compartments, cells were stained with LysoTracker Red DND-99 (Thermo Fisher Scientific) according to the manufacturer's instructions

Abbreviations CHX: Cycloheximide COPD: Chronic obstructive pulmonary disease CSE: Cigarette smoke extract DAMP: Danger-associated molecular pattern IL: Interleukin LPS: Lipopolysaccharide NLR: NOD-like receptor NOD: Nucleotide-binding oligomerization PAMP: Pathogen-associated molecular pattern SDS-PAGE: Sodium dodecyl sulfatepolyacrylamide gel electrophoresis TCA: Trichloroacetic acid References Chaput C, Sander LE, Suttorp N, Opitz B

States approaches to income taxes vary in other details as well

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