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The low and high-speed centrifugation steps were repeated, and the final pellet was resuspended in 0.1 mL CP buffer

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Discussion The prevalence of cigarette sharing (defined as proactively offering cigarettes) among Chinese adults in 2022 is 21.2%, significantly lower than previous reports.4 5 19 This discrepancy stems from methodological variations: (1) Exclusion of passive acceptance versus inclusive approaches in previous studies,4 19 (2) 30-day recall period versus extended time frames (lifetime/past year/past 6 months)5 20 21 and (3) Nationally representative sampling versus region-specific investigations,4 5 20 though actual sharing behavioural declines may also contribute