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Available safety information comes primarily from preclinical animal studies, in vitro research, theoretical extrapolations from full-length IGF-1 and insulin biology, and anecdotal reports from non-clinical use in bodybuilding and performance enhancement contexts - none of which provide the comprehensive, controlled data needed to establish true safety parameters

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doi: 10.1038/s41588-022-01024-z 122 NovoaCSalazarPCisternasPGherardelliCVera-SalazarRZolezziJMet al

FIGURE 4 3.4 Nitazoxanide reduced apoptosis in rats with MTX-hepatotoxicity MTX significantly induced hepatic apoptosis as demonstrated by increased proapoptotic markers, Bax level, and caspase-3 activity (Figures 5A, D) while reduced antiapoptotic Bcl-2 level (Figure 5B) and increased Bax/Bcl-2 ratio (Figure 5C) compared to CTRL group