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Cells were expanded for 2 days and then ExPlus complete growth medium was replaced with PneumaCult ALI-S maintenance medium (StemCell Technologies) in the lower chamber only with the apical chamber exposed to air (ALI day 0)

Ironically, Altria parent company of Marlboro-maker Philip Morris bought a $12.8 billion stake in Juul in 2018, only to break up with the company in 2023

After washing with water, organic molecules on the cartridge column were eluted out using methanol

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