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(2) Comparison of multi-tissue and multi-cell samples: as a metabolic protein, the expression of GAPDH is relatively constant in living tissues, so it is recommended to use GAPDH as an internal reference when comparing multi-tissue and multi-cell samples [6]

Within this pathological network, mtROS and NLRP3 inflammasome activation form a critical molecular hub: mitochondrial dysfunction induces complexes I and III of the electron transport chain to generate large amounts of mtROS, which triggers NLRP3 inflammasome activation through pathways such as promoting the binding of TXNIP to NLRP3, releasing oxidized mitochondrial DNA (ox-mtDNA) as a co-activator, and modulating mitochondrial membrane potential

Zhang Z., Yang X., Song Y