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Following dehydration in ascending concentrations of ethanol solutions (5 min washes in 50%, 70%, 100%, and 100% ethanol), slides were air-dried, and a hydrophobic barrier was created around the sections using a hydrophobic pen (Vector Labs)

Disruption of Slc52a3 gene causes neonatal lethality with riboflavin deficiency in mice

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All animals were verified again using the same protocol after the last experimental day and data from any rat that did not meet verification requirements were removed from the analysis