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Antibiotic and Zn susceptibility testing To determine the MIC of bacterial cells post exposure, cells were grown in drug-free media (not amended with Zn) until saturated (24 h) and subsequently used in a standard broth microdilution MIC in a 96-well plate using LB media ( To test for interactions between Zn and ciprofloxacin that impact the inhibitory concentration, a checkerboard assay was also performed as described by Zn pre-exposure time course and Zn-ciprofloxacin combination assays At the start of the 12-day Zn exposure time-course experiments, saturated liquid cultures were diluted 1:100 in 4 mL of LB broth and grown for 2 h to exponential phase

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