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Although cytosolic CPS2 can produce a cytosolic pool of carbamoyl phosphate, CPS1 is a major rate-limiting enzyme in pyrimidine biosynthesis using nitrogen released via mitochondrial glutaminolysis 32

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The structure of phosphatidylcholine allows it to orient in lipid bilayers, where the hydrophobic fatty acid tails face inward, shielded from the aqueous environment, and the hydrophilic choline-phosphate head groups are exposed to the external and internal water phases, providing stability to the membrane